Then, variants with the desired binding activity are recovered, followed by amplification of the enriched library by reverse transcription–polymerase chain reaction (PCR). 3, 4 During the process, a single-stranded nucleic acid (DNA, RNA, or modified nucleic acids) pool consisting of 10 14–10 15 variants of a random 30–100-nt sequence is incubated with a target molecule. 1, 2Īptamers are identified through an iterative selection–amplification process known as systematic evolution of ligands by exponential enrichment (SELEX). Therefore, the application of aptamers in molecular biology and medical science has been extensively explored. In addition, they can be inactivated under physiological conditions by hybridization with antisense oligonucleotides, and thus, design of an antidote molecule is quite straightforward. Typical aptamers are shorter than 40 nucleotides (nts) and easy for the high-quality production by chemical synthesis. There are, however, several unique qualities of aptamers that are of additional benefit, including robustness against both reducing conditions and heat denaturation. 1, 2 Similar to antibodies, aptamers have high affinity and specificity to target molecules. Here, I review the progress of Cell-SELEX technology and discuss advantages of the technology.Ī ptamers are molecules identified from large combinatorial nucleic acid libraries by their high affinity to target molecules. In addition, cell-specific aptamers can be obtained without any knowledge about cell surface molecules on the target cells. For example, generated aptamers are functional with a native conformation of the target molecule on live cells, and thus, cell surface transmembrane proteins would be targets even when their purifications in native conformations are difficult. ![]() This technology, Cell-SELEX, has several advantages. Although SELEX is typically carried out using purified target molecules, whole live cells are also employable as selection targets. Aptamers are identified through an iterative selection–amplification process known as systematic evolution of ligands by exponential enrichment (SELEX). Due to a variety of desired properties, aptamers are attractive alternatives to antibodies in molecular biology and medical applications. Aptamers are molecules identified from large combinatorial nucleic acid libraries by their high affinity to target molecules.
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